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Liu Lab Equipment List & Research Environment

Our laboratory is on the top floor of the BSE building, an $84 million 227,000 square feet, a five-story structure built 14 years ago. Our laboratory room numbers are BSE 0.110 (EPR), 4.204 (rRaman), 4.314 & 4.320, and our offices at 4.206 and 4.324. Our lab has received the Excellence in Laboratory Safety in the UTSA Gold Star Lab Safety Program and Outstanding Commitment to Laboratory Safety Culture award.

 

Within the Liu laboratory at UTSA, our group members have access to the following major equipments:

 

  • Bruker E560 EPR/ENDOR spectrometer with a cryogen-free 4 K temperature system

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  • Resonance Raman with Innova 302C Kr+ laser from Coherent Inc., PIXIS 1340x400BR Camera, and IsoPlane 320 Spectrograph from Teledyne Princeton Inc.


  • Michrom Gemini microcapillary LC interfaced with a Thermo-Finnigan LCQ ESI mass spectrometer with nanospray (LC-MS/MS)


  • Applied Photophysics SX20 Stopped-Flow UV-Vis spectrophotometer with a photomultiplier tube, and photodiode array as well as fluorescence detectors; Rapid-freeze quench (RFQ) apparatus (from Update Instruments), and Agilent 8453 UV-visible spectroscopy system


  • The synthetic corner: Teledyne Isco CombiFlash Rf+ chromatography, IKA rotovapor, chiller, and vacuum gas manifold


  • Coy glove and gloveless anaerobic chamber


  • Thermo Scientific Ultimate-3000SD UHPLC rapid separation system with an autosampler, a photodiode array detector, and a fluorescence detector


  • Microcal isothermal calorimeter (VP-ITC)

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  • Gryphon crystallization robotic system from Art Robbins Instruments

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  • Leica microscope, vibration-free crystallization refrigerators, and Linux workstations for remote data collection at the beamlines


  • Thermo Scientific ISQ EC Single Quadrupole Mass Spectrometer interfaced with HPLC


  • Tecan Infinite M200 Quad monochrometer microplate UV-vis and fluorescence spectrometer for the screening of therapeutic compounds


  • New Brunswick Scientific BioFlo 410 fermentor (already upgraded to model 415 in picture not updated)


  • Microfluidics LM20-30 cell disruptor

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  • AKTA FPLC, AKTA purifier, and two AKTA start systems


  • Cary eclipse fluorescence spectrometer and homemade O2 level controller


  • Refrigerated shakers from Eppendorf Scientific (New Brunswick) and Thermo


  • Multiple -80 C freezers and refrigerators


    • Not pictured equipment:

  • Mammalian cell line culture including Nuaire CO2 water-jacketed incubator (it is currnetly lent to a colleague)
  • All relevant tools for protein crystallography, including Linux workstations and Molecular Dimensions vibration-free refrigerators
  • Clark-type oxygen electrode and hydrogen peroxdie oxygen electrode with upgraded gas blender
  • WPI free radical analyzer with hydrogen peroxide electrode
  • Update Instruments Model 715 rapid mixer and quenching system (RFQ)
  • Agilent/Hewlett-Packard 8453 diode array spectrophotometers (2X)
  • Perkin-Elmer Lambda 25 spectrophotometer
  • Turner design/Promega model TD-20/20 luminometer
  • Leitz HM-Lux microscope
  • Olympus CKX41SF2, inverted stage, phase contrast microscope with camera
  • Perkin Elmer thermal cycler (block type) and Eppendorf mastercycler gradient PCR systems
  • Class II Type A2 biosafety cabinet
  • Beckman high-speed centrifuges: Avanti J-26 XP & J-E
  • Anaerobic Shlenk line
  • Speed vac and lyophilizer
  • Alpha Mager HP

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    Research Environment

     

     

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